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Western blot analysis of surface extracts of E. faecalis OG1SSp(pMSP7517) with antiserum raised against recombinant AS44–331. Wild-type AS rapidly degrades when released from the cell, and multiple bands are common. Lane 1, uninduced culture (Asc10 negative); lane 2, Asc10 induced with 25 ng of nisin/ml. Arrows indicate bands corresponding to fragments of the Asc10 protein.
