Table 1.
1H chemical shift (δH, ppm) values of α-anomerics (1H1) of branching Fucs (A-D), acetyl methyl groups of GalNAc, and fucosyl methyl groups from PpFucCS and oligosaccharides
| δH (ppm)a | |||||
|---|---|---|---|---|---|
| Unit | PpFucCS | HdPpFucCS | Fr1 | Fr2 | Fr3 |
| Fuc2,4S (A) | 5.83 | 5.84 | 5.85 | 5.86 | 5.84 |
| Fuc2,4S (B) | 5.76 | 5.68 | 5.69 | 5.70 | 5.68 |
| Fuc4S (C) | 5.64 | 5.56 | 5.57 | 5.57 | 5.55 |
| Fuc0S (D) | 5.59 | 5.50 | 5.52 | 5.52 | 5.50 |
| GalNAc-CH3 | 2.30 | 2.22 | 2.20 | 2.24 | 2.23 |
| Fuc-CH3 | 1.50 | 1.51 | 1.52 | 1.52 | 1.52 |
a
Chemical shift values were acquired at 500 MHz at 50 °C and referenced to external trimethylsilylpropionic acid for 0 ppm for 1H.