Table 2.
IC50 values of anticoagulant and anti-SARS-CoV-2 Delta (B.1.617.2) activities of UFH, PpFucCS, and PpFucCS oligosaccharides
| Glycan | Anticoagulant activity | Anti-SARS-CoV-2 (B.1.617.2)a | ||||
|---|---|---|---|---|---|---|
| aPTT (IU/mg)b | HCII/IIa | AT/Xa | AT/IIa | IC50 (μg/mL) | Imax | |
| IC50 (μg/mL) | ||||||
| UFH | 180 | 0.6 ± 0.07 | 0.025 ± 0.06 | 0.03 ± 0.003 | 0.3 ± 0.2 | 98.4 ± 0.8 |
| PpFucCS | 30.2 ± 0.02 | 0.2 ± 0.02 | 1.8 ± 0.2 | 1.2 ± 0.07 | 0.5 ± 0.3 | 98 ± 0.2 |
| HdPpFucCS | 6.6 ± 0.44 | 9.6 ± 1.3 | NDc | ND | 5.4 ± 1.4 | 94.7 ± 2.7 |
| Fr1 | 12 ± 0.19 | 4.7 ± 0.8 | ND | ND | 3 ± 0.2 | 96.5 ± 1.0 |
| Fr2 | ND | ND | ND | ND | 0.2 ± 0.2 | 75.8 ± 4.5 |
| Fr3 | ND | ND | ND | ND | 0.3 ± 0.5 | 56 ± 8.6 |
| Fr4 | ND | ND | ND | ND | ND | 28 ± 15.5 |
| CS-B | - | 6.2 ± 2.1 | - | - | - | - |
a
IC50 values of anti-SARS-CoV-2 inhibitory activity of UFH, PpFucCS, and PpFucCS oligosaccharides were determined against HEK 293T-hACE2 cells infected with baculovirus pseudotyped with SARS-CoV-2 S-protein of the Delta variant. Each value is mean ± SD of triplicate measurements.
b
Values were calculated using a parallel UFH (180 IU/mg) standard curve. Each value is mean ± SD of triplicate measurements.
c
ND, not determined.