Table 3.
Inhibitory percentage of exogenous sulfated glycans in competitive SPR binding of coagulation Serpins (AT and HCII), factor IIa, and SARS-CoV-2 S-protein RBDs (WT and variants) to heparin-immobilized sensor chip
| Glycan | Blood (co)-factors | SARS-CoV-2 S-protein RBDs | ||||||
|---|---|---|---|---|---|---|---|---|
| AT | HCII | IIa | WT | N501Y | Delta | Triple mutant | Omicron BA.2 | |
| UFH | 95 ± 0.7 | 100 | 75 ± 0.3 | 93 ± 2.5 | 93 ± 2.6 | 81 ± 4.5 | 100 | 54 ± 6.7 |
| PpFucCS | 34 ± 2.2 | 100 | 55 ± 0.4 | 100 | 100 | 100 | 100 | 85 ± 3.8 |
| HdPpFucCS | 8 ± 0.3 | 100 | 56 ± 0.8 | 100 | 98 ± 1.5 | 71 ± 7.2 | 100 | 70 ± 1.7 |
| Fr1 | 10 ± 1.6 | 100 | 55 ± 0.6 | 96 ± 1.2 | 91 ± 3.7 | 77 ± 8.1 | 99 ± 1.6 | 67 ± 1.9 |
| Fr2 | 6 ± 1.4 | 100 | 35 ± 0.5 | 71 ± 5.6 | 61 ± 9.5 | 60 ± 8.7 | 80 ± 3.7 | 61 ± 2.9 |
| Fr3 | 8 ± 0.5 | 90 ± 8.9 | 55 ± 0.6 | 46 ± 5.9 | 43 ± 11.9 | 60 ± 7.6 | 49 ± 3.2 | 52 ± 3.8 |
| Fr4 | 3 ± 1.2 | 77 ± 6.8 | 20 ± 0.7 | 25 ± 6.3 | 35 ± 14.6 | 60 ± 4.6 | 36 ± 3.7 | 31 ± 4.3 |
Values in the table were calculated by subtracting the normalized binding values (in %) from the control value (100%) which are just proteins alone. Normalized binding values were determined from competitive SPR measurements of the binding S-proteins and coagulation factors (serpins and protease) to surface heparin in the presence of UFH, PpFucCS, and PpFucCS oligosaccharides. Each value is mean ± SD of triplicate measurements.