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. 2022 Dec 27;18(12):e1011066. doi: 10.1371/journal.ppat.1011066

Fig 3. Growth inhibitory effects exerted by effluxed fluoropyrimidines on mutants lacking key pyrimidine salvage activities.

Fig 3

(A) Evaluation of the supernatants’ impact on the colony growth of strains on solid medium. (B) Microtiter-based hyphal growth quantification and (C) representative microscopic images illustrating growth of strains in liquid AMM supplemented with distinct supernatants. (D) Prediction of the fluoropyrimidine content in supernatants of the respective strains. The susceptibility displayed by ΔuprtΔurkA grown in the presence of wt and Δuprt supernatants suggests the efflux of 5FUMP and/or further toxic fluoropyrimidines (5FPs). Scale bars: (A) 1 cm, (C) 100 μm.