Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2022 Dec 21;3(1):4. doi: 10.3892/mi.2022.64

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright: © Kubota et al.

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, reproduction and adaptation in any medium and for any purpose provided that it is properly attributed. For attribution, the original author(s), title, publication source (PeerJ) and either DOI or URL of the article must be cited.

PMC Copyright notice

Western blot analysis. Escherichia coli BL-21 cells containing the pGEX-4T-1-TSTD2 were treated without (lane 1) or with 0.1 mM isopropyl β-D-1-thiogalactopyranoside (lane 2) for 3 h at 42˚C in Luria broth. The whole cell extract was electrophoresed with sodium dodecyl sulfate-polyacrylamide (11%) gels followed by staining with CBB. The asterisk represents GST-tagged full-length TSTD2 (GST-TSTD2) protein in the whole cell extract. Affinity-purified GST (lane 3) and GST-TSTD2 proteins (lane 4) were electrophoresed similarly and then stained with CBB or used for western blotting with anti-GST antibodies or the serum antibodies of a patient with aCI and those of a HD. Electrophoresed molecular weight markers are also shown in lane M, with the sizes shown on the left. CBB, Coomassie Brilliant Blue; GST, glutathione-S-transferase; aCI, acute cerebral infarction; HD, healthy donor.