Extended data Figure 7.

related to Figure 5. a, experimental design for forced expression of Pou2af1, the gene encoding OCAB in mouse. Splenic B cells from n=5 mice were stimulated with LPS ex vivo and transduced with a GFP vector containing Pou2af1 (mOCAB) or GFP only (control). b, expression of Pou2af1 in mOCAB (n=5) and control(n=5) B cells. Expression levels were measured by qPCR using cDNA generated from total RNA of GFP+ viable cells and normalized to that of Hprt1, calculated by the delta-delta Ct method (2^−∆∆Ct) and represented as a fold change over the mean expression in control cells. The expression of each gene was measured in triplicate across 5 replicates. P-values by Welch’s t tes and error bars show standard error of the mean.