Figure 7.

Turnover of SLC30A10-WT and SLC30A10-T95I is comparable. A: HepG2 clones overexpressing SLC30A10-WT or SLC30A10-T95I (identical to Fig. 4) were treated with 0.5 µM MG132 for indicated times. Cell lysates were collected and SLC30A10 or tubulin were detected by immunoblot. The custom antibody against SLC30A10 was used. B: quantification of SLC30A10 levels from A. At each time point, SLC30A10 expression is normalized to tubulin. For each infection condition, normalized SLC30A10 expression at 0 h is set to 1 (n = 3; there were no differences between SLC30A10-WT or SLC30A10-T95I groups at any time-point using two-way ANOVA and Sidak’s post hoc test). WT, wild type.