FIG. 1.

Levels of B. burgdorferi and the HGE agent after 1 week of coinfection. DNAs from specific tissues were pooled from five mice (from each group of animals) and analyzed by PCR with primers specific for either B. burgdorferi ospA or hge-44. Bladder (A) and skin (B) DNAs from singly B. burgdorferi-infected (lanes 1) and coinfected (lanes 2) mice were analyzed for ospA. Blood (C), joint (D), heart (E), and splenic (F) DNAs were analyzed by dilutional PCR for hge-44. The numbers represent concentrations of total DNA used in the PCR. PCR for β-actin was performed with 1 μg of total DNA to ensure that equal amounts of DNA were used. These data represent samples from one experiment with five mice in each group. Five separate experiments with different mice yielded similar results.