Extended Data Fig. 8. CRISPR Screen additional information SEC24B disease association and flow cytometry.
a, intersection of hits from various published CRISPR and siRNA screens. b, Details on cell type, treatment, and screen approach for various published works. C and d, Scatter plots of overlapping hits from the two viral pools in 8 hr and 24 hr treatments. Statistical analysis performed by MAGeCK. Genes highlighted in purple were identified in previously published ferroptosis CRISPR screens. e, Lipid peroxidation as defined by percent of FITCbright PE+ cells in isogenic and SEC24B KO lines when treated with vehicle (n = 6 biologically independent experiments). One-tailed paired t test. p-values are indicated in graph. f, Western blot for P62 in isogenic and SEC24B KO HAP1 cell lines serum-starved and treated with vehicle or 100 µM chloroquine (CQ) for 6hrs. g, Quantification of western from f (n = 3 biologically independent experiments). One-way ANOVA, tukey post-hoc. p-values are indicated in graph. Error bars represent SEM. h, Expression of SEC24B in multiple neurodegenerative diseases.