TABLE 2.
Primers used for expression of recombinant PspA fragmentsa
| Primer | Position in sequence (bp) | Sequence |
|---|---|---|
| ABW23 | 400–417 | 5′-TCTGATATTCAGCGTCAG-3′ |
| HR10 | 428–447 | 5′-GCATATAAAGAGTACCGAGA-3′ |
| HR11 | 1103–1121 | 5′-GATACTGCTGCTCTTCCAAA-3′ |
| HR14 | 1656–1675 | 5′-CTACTCAACCAGAAAAACCA-3′ |
| LSM12 | 149–173 | 5′-AGCGTCGCTATCTTAGGGGCTGGTT-3′ |
| LSM150 | 577–591 | 5′-CCAAGGATCCGTGGATGCTGAAGAA-3′ |
| LSM16 | 928–942 | 5′-CGCGTCGACACTCTCATTAACTGCTTT-3′ |
a
Primers LSM150 and LSM16 were designed from the pspA sequence of S. pneumoniae Rx1, and the remaining primers were from the pspA sequence of S. pneumoniae EF3296.