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Shake flask fermentation of 20 candidate thermophilic strains for thermophilic enzyme GNA1 performance tests
(A−D) The construction of five gene-knockout plasmids (A). Shake flask fermentation of 20 candidate thermophilic strains at 37°C (B), 42°C (C), and 50°C (D). Triplicate experiments were done for physiological measurements, and error bars represent SD.
