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Detection of Cox-1 and Cox-2 in uninfected and E. histolytica-infected human intestinal xenografts by Western blotting. A total of 106 E. histolytica HM1:IMSS trophozoites (106/100 μl) were injected into the lumens of xenografts. Host mice were killed 24 h later, and the human xenograft tissue was harvested and homogenized. Cox-1 and Cox-2 proteins were detected using rabbit anti-sheep antibodies. Purified sheep Cox-1 and sheep Cox-2 proteins served as positive controls.
