TABLE 1.
Serological characteristics of MAbs 12A1 and 13F1 and the 12A1→13F1 mutants
| Antibody | Reactivity by direct ELISAa | Inhibition with MAb 3E5b | Reactivity with anti-idiotypic MAb 7B8c | Phagocytic indexd | Immunofluorescence |
|---|---|---|---|---|---|
| 12A1 | +++ | Yes | + | +++ | Annular |
| 13F1 | + | No | − | − | Punctate |
| 12A1VH-12A1VL | +++ | Yes | + | +++ | Annular |
| 12A1VH-13F1VL | +++ | Yes | − | +++ | Annular |
| N31S | +++ | Yes | + | + | Annular |
| F33Y | ++ | Yes | + | + | Annular |
| M50A | +++ | Yes | + | ++ | Annular |
| I53S | +++ | Yes | + | ++ | Annular |
| N56G | +++ | Yes | + | ++ | Annular |
| N57S | ++ | Yes | + | + | Annular |
| D80Y | +++ | Yes | + | ++ | Annular |
| F33Y N57S | + | No | − | − | Punctate |
a
Plus signs indicate the relative reactivity of the Ab for GXM as measured by direct ELISA, ranging from weak (+) to strong (+++).
b
For representative data, see Fig. 5.
c
+, reactivity; −, lack of reactivity. Data are in Fig. 7.
d
Plus signs indicate the relative opsonic efficacies of these MAbs; minus signs indicate absence of or minimal opsonic efficacy. For details of phagocytic indices observed with MAbs 12A1 and 13F1, see reference 11.