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. 2023 Jan 9;14:116. doi: 10.1038/s41467-022-35741-0

Fig. 5. Phosphorylation of Pg at S665 is important for desmosomal adhesion in murine epidermis.

Fig. 5

A Introduction of S665A mutation into the plakoglobin (JUP) locus. The phospho-site of interest is located at S665 in the C-terminal tail region. B, C Immunostaining for Dsg1 (wt: n = 4, Pg-S665A: n = 7) and Dsg3 (n = 3) was diminished in Pg-S665A mice. D Dsc1 and CK14 reveal a differentiation-dependent staining pattern in wt and are almost absent in Pg-S665A mice (n = 5). E Pg is reduced along cell borders in Pg-S665A (n = 6) mice compared to wt (n = 5). F pPg-S665 was present in a dotted fashion along cell borders and in the nuclei in wt (n = 3) but not in Pg-S665A (n = 6) epidermis. White rectangles represent areas for zoom in. Scale bar (zoom) = 8 µm. B–F White dotted lanes represent basement membranes. Representatives of n ≥ 3. Scale bar = 25 µm. G HE staining of wt and Pg-S665A neonatal epidermis revealed no significant changes in epidermal morphology or thickness in untreated conditions. In contrast, in Pg-S665A but not in wt mice blisters occurred after exposure to mechanical stress. Representative of n = 6. Scale bar = 25 µm. H Quantification of HE after application of mechanical stress. Columns indicate mean value ±SEM. *P = 0.022. Two-tailed Student’s t test. Source data are provided as a Source Data file.