Fig. 6. Validation of the IRE1a signature and RNA-seq deconvolution.

a Violin plot of enrichment scores (ssGSEA) for IRE1α^KO gene signature (Log2 fold-change >1 and FDR 0.01) low (n = 15, blue), mid (gray, n = 14), and high (red, n = 15) NSCLC patients. One-way ANOVA with Tukey’s multiple comparisons test for ratios. b Kaplan–Meier survival plots depicting associations between overall survival (OS) and IRE1α^KO signature status of NSCLC patients stratified into the top and bottom tertiles (15 patients in each) for the IRE1a^KO gene signature. c–n Violin plots of xCell pipeline enrichment scores for microenvironment (c P < 0.0001), immune (d P = 0.001), stromal (e P = 0.001), dendritic (f P = ns), CD4 + T cells (g P = ns), CD4 + naïve (h P = 0.0027), CD4 memory (i P = ns), T-regulatory (j P = NS), CD8 + T cell (k P = ns), CD8 + Naïve (l P = ns), CD8 + TCM (m P = ns), and CD8 + effector memory (n P = ns) from the validation set of lung cancer patients from the IRE1α signature high (red, n = 15) and low (blue, n = 15) groups for the enrichment of various immune cells. Unpaired, two-tailed, Student’s t-test. *P < 0.05, **P < 0.001, and ***P < 0.0001. o, p IF analysis showing quantitation of CD3T cells (left panel) and Tregs (right panel) in tumor nests of validation set of lung cancer patients from the IRE1α signature high (red, n = 30) and low (blue, n = 30) groups. Unpaired, two-tailed, Student’s t-test. *P < 0.05, **P < 0.001, and ***P < 0.0001. Source data are provided as a Source Data file.