Figure 5.

Knockout of TF Msn2/Msn4 does not influence the main results of enhanced stress response under glucose-limited conditions

(A) Normalized growth curves of the different strains after the 0.8 M KCl stimulation was added, showing the average number of cells relative to the number of cells before stress. Yeast grew in 2% glucose. Error bars are SD from three independent experiments. In each experiment, cell numbers were 90∼100.

(B) Normalized growth curves of the different strains after the 0.8 M KCl stimulation was added, showing the average number of cells relative to the number of cells before stress. Yeast grew in 0.1% glucose.

(C) Localization trajectory of nuclear Hog1 in the wild type and msn2Δmsn4Δ strain. In total, 0.8 M KCl was added at 3 h. The curve represents a single cell whose nuclear duration is the median value in the population. The numbers of cells of msn2Δmsn4Δ strain included in the analysis of the 2% and 0.1% glucose concentrations were 25 and 22, respectively. Wild-type data are derived from main text Figure 2.

(D) The duration of Hog1 nuclear localization of wild type and msn2Δmsn4Δ strain. Data are represented as mean +/− SEM. Statistical significance is calculated with Student’s t test (∗∗p < 0.01, ∗∗∗p < 0.001).

(E) The expression of Gpp1 (Hog-MAPK-dependent protein), Hsp12 (both Hog-MAPK- and Msn2/4-dependent protein), and Hxk1 (Msn2/4-dependent protein) upon osmotic stress under different glucose concentrations. 0.8 M KCl was added at 2h. The curve represents a single cell whose intensity is the median value in the population of 30∼50 cells.

(F) Bar graph of the increase ratio of protein response intensity to osmotic stress under different glucose environments (maximum response value minus initial value divided by initial value). Data were from Figure 5E and represented as mean +/− SEM.