Alpha diversity and taxonomic composition of intestinal content (CT, n = 4; HFD, n = 5; EMPA, n = 5)
(A and B) Differences in gut microbiota diversity among the groups of mice were estimated by the Simpson (A) and Shannon (B) indices.
(C–F) (C) Principal component analysis plot of gut microflora species determined from metagenomic sequencing of the three groups. Comparison of the average abundance of certain gut bacteria from mice in CT, HFD, and EMPA groups at the species (D), genus (E), and phylum (F) level. Data are represented as mean ± SEM.