Figure 2.

Rosiglitazone and insulin are the essential components of the differentiation cocktail

(A) Illustration of the experimental design for 2b. Bovine SVC cells were proliferated in serum-free growth medium (SFGM) and differentiated with a defined animal component-free medium (DMAD). Varying concentrations or removal of adipogenic inducers: IBMX/dexamethasone (Dex) were tested during the induction phase; rosiglitazone (Ros)/insulin (Ins) were tested during the induction and progression phase.

(B and C) Quantification of lipid accumulation with HCA shown at day 8. (B) Concentration gradients of differentiation media components were tested at 0, 25 and 400% of their standard concentrations. (C) Varying sequences of induction (I), progression (P) and maintenance (M) media. -ctrl, no inducers; I→P or control differentiation medium (cDM) has all 4 inducers in the first media change followed by Ros and Ins (P); P→P or reduced differentiation medium (rDM) contains only Ros and Ins as inducers. Data are represented as mean ± SD; the error bars represent the SD of 4 independent experiments using 4 donors. Statistical analyses and comparisons were performed using a one-way ANOVA; NS, not significant; ∗p < 0.05; ∗∗p < 0.01; ∗∗∗p < 0.001; and ∗∗∗∗p < 0.0001.

(D) Representative images taken on day 8. Blue, Hoechst; and green, BODIPY. Scale bar, 100 μm. See also Figure S1.