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. 2022 Dec 17;26(1):105822. doi: 10.1016/j.isci.2022.105822

Figure 3.

Figure 3

Rosiglitazone/insulin and hydrocortisone/progesterone are necessary for high quality differentiation

(A) Illustration of the experimental design. Bovine SVC were proliferated in SFGM and differentiated with DMAD. Rosiglitazone and insulin were removed from the reduced differentiation media (rDM). Hydrocortisone and progesterone (HC/PR) were also excluded because they are reported to activate GCR, same as dexamethasone.

(B) Screening the necessity of rosiglitazone (Ros) and insulin (Ins) within rDM. Adipogenic quantification at day 8.

(C) HC/PR were excluded from rDM or control differentiation media (cDM). In cDM-HC/PR, IBMX was present during the first 4 days, and dexamethasone was absent entirely. (B and C) Data are represented as mean ± SD; the error bars represent the SD of 4 independent experiments using 4 isolations. Statistical analyses were performed using a one-way ANOVA; ∗p < 0.05; ∗∗∗p < 0.001; and ∗∗∗∗p < 0.0001.

(D) Representative images taken on day 8. Blue, Hoechst; and green, BODIPY. Scale bar, 100 μm. See also Figure S2.