Figure 6.

Transcriptomic analysis demonstrates the impact of insulin, rosiglitazone and glucocorticoid receptor binding molecules on differentiating adipocytes

(A-C) Bovine SVC were proliferated in SFGM and differentiated with DMAD rDM, rDM 0% Ins, 0% Ros or 0% HC/PR. mRNA was harvested at day 12. 3 donors were used in 3 independent experiments.

(A) Heatmap showing the Z score normalized expression values of all 894 genes that were differentially expressed in at least one comparison.

(B) Venn diagram showing overlapping GO terms corresponding to upregulated genes in rDM when compared to rDM deprived from insulin, rosiglitazone, or HC/PR.

(C) Selection of differentially expressed genes of indicated GO terms. Fatty acid synthase (FASN) is involved in FA synthesis (metabolic process); Cell death activator CIDE-A (CIDEA) involved in lipid droplet enlargement (lipid storage); ELOVL6 plays a role in very long chain fatty acid elongation; Stearoyl-CoA desaturase (SCD) is involved in the generation of unsaturated FA; Apolipoprotein E (APOE) is involved in cholesterol metabolism and fat intratissue transportation; and Cytochrome c oxidase subunit 6A1 (COX6A1) is involved in oxidative phosphorylation. Data are represented as mean ± SD. Statistical analyses and comparisons were performed using a two-way ANOVA; NS, not significant; ∗p < 0.05; ∗∗p < 0.01; ∗∗∗p < 0.001; and ∗∗∗∗p < 0.0001. See also Figure S7.