Figure 7.

Adipogenic differentiation of different species: mouse 3T3-L1 cell line; porcine FAPs (monogastric); and sheep FAPs (ruminant)

Differentiation with control (cDM in 3%FBS) or reduced (rDM in DMAD) differentiation medium at day 28. Cells were encapsulated with alginate hydrogel and differentiated with 3%FBS or DMAD. 3%FBS cDM contained all 4 inducers during first media change followed by progression for the whole period of culture, rDM contained rosiglitazone and insulin for 28 days. DMAD contained HC/PR. n = 3 donors. Representative images of maximum intensity projection confocal microscopy (Blue, Hoechst; and yellow, Nile Red). Scale bar, 100 μm.