REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
anti-rabbit Alexa 594	Thermo Fisher	Donkey rAb Cat #R37119; RRID:AB_2556547
anti-mouse ACACA	Cell signaling technology	Rabbit mAb Cat #3676; RRID:AB_2219397
anti-mouse CEBPɑ	Cell signalling technology	Rabbit mAb Cat #8178; RRID:AB_11178517
anti-mouse PLIN1	Cell signalling technology	Rabbit mAb Cat #9349; RRID:AB_10829911
anti-mouse PPARγ	Cell signalling technology	Rabbit mAb Cat #2435; RRID:AB_2166051
Biological samples
Bovine subcutaneous adipose tissue	Local abattoir	N/A
Porcine semitendinosus muscle	Local abattoir	N/A
Ovine semitendinosus muscle	Local abattoir	N/A
Chemicals, peptides and recombinant proteins
DMEM/F12	Gibco	Cat# 21331-020
DMEM	Gibco	Cat# 41966-029
PSA	Lonza	Cat# 17-745E
FBS	Gibco	Cat# 10500-064
Trypsin-EDTA	Gibco	Cat# 25300-062
Glutamine	Gibco	Cat# 35050-061
Lipid concentrate	Gibco	Cat# 11905-031
HEPES	Sigma-Aldrich	Cat# H4034
Putrescine	Sigma-Aldrich	Cat# 51799
Progesterone	Sigma-Aldrich	Cat# P8783
Hydrocortisone	Sigma-Aldrich	Cat# H0135
Ascorbic acid	Sigma-Aldrich	Cat# A8960
BMP4	Peprotech	Cat# 120-05ET
FGF2	Peprotech	Cat# 100-18B
EGF	Peprotech	Cat# AF-100-15
Insulin	Sigma-Aldrich	Cat# I0516
Rosiglitazone	Sigma-Aldrich	Cat# R2408
Dexamethasone	Sigma-Aldrich	Cat# D1756
IBMX	Sigma-Aldrich	Cat# I5879
Alginate	Sigma-Aldrich	Cat# W201502
Bodipy	Thermo Fisher	Cat# D3922
Hoechst 34,580	Sigma-Aldrich	Cat# 63493
Nile-red	Sigma-Aldrich	Cat# N3013
Collagenase	Worthington	Cat# CLSAFA
CaCl2	Sigma-Aldrich	Cat# C3881
iQ SYBR Green Supermix	Bio-Rad	Cat# 1708880
PBS	Gibco	Cat# 10010023
Critical commercial assays
E.Z.N.A Total RNA kit II	Omega Bio-tek	Cat# R6934
iScript cDNA synthesis kit	Bio-Rad	Cat# 1708891
Dynabeads mRNA DIRECT™ MicroKit	Thermo Fisher	Cat# 61021
PCR-cDNA Barcoding kit	Oxford Nanopore Technologies	Cat# SQK-PCB109
Deposited data
RNA sequencing	This study	GEO: GSE206990
Experimental models: Cell lines
3T3-L1	Green et al., 1974	RRID:CVCL_0123
Oligonucleotides
Table S2	Eurogentec	N/A
Software and algorithms
GraphPad Prism 9.1.0	GraphPad Software	https://www.graphpad.com/scientific-software/prism/
Inkscape	Inkscape software	https://inkscape.org/
Guppy 5.0.16	Oxford Nanopore Technologies	nanoporetech.com
Minimap2	https://github.com/lh3/minimap2	N/A
Salmon	https://github.com/COMBINE-lab/salmon	N/A
DEseq2	https://bioconductor.org/packages/release/bioc/html/DESeq2.html	N/A
tximport	https://bioconductor.org/packages/release/bioc/html/tximport.html	N/A
SVA	https://bioconductor.org/packages/release/bioc/html/sva.html	N/A
Piano	https://bioconductor.org/packages/release/bioc/html/piano.html	N/A