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. 2023 Jan 1;19(2):691–704. doi: 10.7150/ijbs.79205

Figure 5.

Figure 5

NSUN2 exerts a tumor-promoting role in CC. (A) CCK-8 assays showed the growth of HeLa cells upon NSUN2 overexpression. (B) The effect of NSUN2 overexpression on the colony formation of HeLa cells. (C) Transwell cell migration and invasion analysis of NSUN2 overexpressed and control HeLa cells. Scale bar, 200 μm. (D) The effect of NSUN2 knockdown on cell growth was determined by CCK-8 assays. (E) Colony formation assays were performed in NSUN2 knockdown and control cells. (F) Migration and invasion assays of HeLa cells with or without NSUN2 knockdown. Scale bar, 200 μm. (G-I) Effects of NSUN2 knockdown on tumor weight and volume in the subcutaneous xenograft nude mouse model. (J-L) Representive IHC staining images and quantative analysis of Ki67 and Caspase-3 in xenograft tumors from NSUN2 knockdown and control cells treated nude mice. Scale bar, 500 μm. Statistical analyses showed the IHC staining of Ki67 and Caspase-3 from xenograft tumors. Data are presented as means ± S.D: ***P < 0.001.