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. 2023 Jan 1;19(2):691–704. doi: 10.7150/ijbs.79205

Figure 6.

Figure 6

NSUN2 facilitates tumorigenesis of CC cells in an m5C-dependent manner. (A) Schematic of the NSUN2 mutant (NSUN2-MUT). (B) Western blot confirmed the overexpression of wild-type or mutated NSUN2 in CC cells. (C) CCK-8 assays of SiHa cells with forced expression of wild-type or mutated NSUN2. (D) Colony formation assays were performed in SiHa cells with forced expression of wild-type or mutated NSUN2. (E) Transwell cell migration and invasion assays of SiHa cells with NSUN2-WT and NSUN2-MUT. Scale bar, 200 μm. (F, G) Cumulative proportion of NSUN2 bound transcripts with differentially expressed genes from RNA-seq analysis of NSUN2 knockdown in HeLa and SiHa cells. (H) Effects of NSUN2 knockdown on mRNA half-life of LRRC8A by RNA stability assays. (J, K) RT-qPCR detecting RNA levels of LRRC8A in HeLa and SiHa cells upon YBX1 knockdown. (I) RIP-PCR assays detecting the interactions between YBX1 and LRRC8A mRNA in HeLa and SiHa cells. IgG was used as an internal control. GAPDH was used as the negative control in western blot assays. Data are presented as means ± S.D: ***P < 0.001.