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. 2023 Jan 1;19(2):362–376. doi: 10.7150/ijbs.69784

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FCN3 interacted with SBDS and regulated the nuclear import of EIF6 and ribosome biogenesis. (A) COG class annotation results of all identified proteins by Co-IP MS assay. (B) FCN3-binding proteins in the Co-IP MS results and top 13 score of proteins were shown. (C) Exogenous Co-IP assay in HEK-293T cells co-transfected with His-FCN3 and cMyc-SBDS. (D) Endogenous Co-IP assay in HCCLM3 and SK-Hep1 cells transfected with His-FCN3. (E) FRET assay in living HCCLM3 cells (FRET-Efficiency= (Donor_post-Donor_pre)/ Donor_post). (F) Western blot of EIF6 nucleocytoplasmic separation in HCCLM3 and SK-Hep1 overexpressing FCN3. (G) Western blot of EIF6 nucleocytoplasmic separation in HepG2 cells downregulated FCN3. (H) Western blot of RPL5, RPL11, RPL23 and MDM2 in HCCLM3 and SK-Hep1 cells transfected FCN3. (I) Co-IP assay with RPL11 antibody in HCCLM3 and SK-Hep1 cells overexpressed FCN3. (J, K) Ribosome profiles at 260nm absorbance in HCCLM3 and SK-Hep1 cells overexpressing FCN3. The location of 40S, 60S and 80S were indicated by arrows. *P<0.05.