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. 2023 Jan 1;19(2):465–483. doi: 10.7150/ijbs.72218

Figure 2.

Figure 2

BGN promotes the proliferation, migration, invasion, and EMT of GC cells. *P < 0.05, **P < 0.01, ***P < 0.001. Data are shown as mean ± SEM (n = 3). a BGN expression was validated in six GC cell lines, the human gastric epithelial cell line (GES1). Three different siRNAs of BGN were designed and transfected into HGC27, and si-3 had the optimal knockdown effect. Lentiviral-based BGN knockdown stable HGC27 cell line (anti-BGN HGC27), lentiviral-based BGN overexpression stable AGS cell line (Oe-BGN AGS), and negative control stable cell lines were verified by western blot. b-j The effect of BGN on the proliferation, migration, invasion, and EMT of GC cells was detected with lentiviral-based BGN overexpression and knockdown stable cell lines by the CCK8 assay (c-d), colony formation assay (e-f), wound healing assay (g-h), Transwell migration and invasion assay (i-j), and western blot analysis (b), respectively. Representative photographs of wound healing (Original magnification 40×), migratory or invaded cells (Original magnification 200×).