FIG. 2.

Binding of FH to PspC from different pneumococcal isolates. Cell lysates were loaded at equivalent protein concentrations onto three gels and separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. (A) Blot stained with Coomassie blue; (B) Western blot reacted with biotinylated FH; (C) Western blot reacted with anti-PspC antibody. Lane 1, E. coli Y1090; lanes 2 to 8, S. pneumoniae strains D39, TRE108, TRE144, LM91, L82013, L81905, and DBL6A, respectively. TRE108 is an isogenic mutant of D39 that lacks PspC. TRE144 has a insertion downstream of the pspC gene in D39. LM91 is an isogenic mutant of D39 that lacks PspA.