Skip to main content
. Author manuscript; available in PMC: 2023 Feb 1.
Published in final edited form as: Nat Microbiol. 2022 Jul 11;7(8):1210–1220. doi: 10.1038/s41564-022-01162-4

Extended Data Fig. 4 |. AvcD-Avci complex formation in solution and Denaturing urea PAGE analysis of Avci and Avci-RC.

Extended Data Fig. 4 |

(A) AvcD forms a complex with AvcI in an AvcD concentration-dependent manner as determined by EMSA. Trace quantities of AvcI reverse complement (AvcI-RC) binding to AvcD is observed. (B) AvcI and AvcI-RC run at essentially equivalent molecular weights on a 7 M urea denaturing PAGE. Low range ssRNA ladder (NEB). This was performed at least three times, yielding similar results.