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. Author manuscript; available in PMC: 2023 Feb 1.
Published in final edited form as: Nat Microbiol. 2022 Jul 11;7(8):1210–1220. doi: 10.1038/s41564-022-01162-4

Extended Data Fig. 9 |. Cessation of global translation, by treatment with spectinomycin, does not liberate AvcD enzymatic activity.

Extended Data Fig. 9 |

Intracellular abundance of dCTP (A), dCMP (B), dUTP (C), and dUMP (D) of WT and ΔavcD V. cholerae during spectinomycin treatment (200 μg/mL) measured by UPLC-MS/MS. Data represent the mean ± SEM of three biological replicate cultures. No statistically significant differences in nucleotide concentrations were observed between strains at any time point as determined by Two-way ANOVA with Two-way ANOVA with Šídák’s multiple-comparison test.