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. 2023 Jan 9;21:3. doi: 10.1186/s12959-022-00443-6

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© The Author(s) 2023

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 2 — Gene cloning and plasmid vector construction. (a) Schematic diagram of mutant c.1377delC plasmid vector: pCDH-CMV-SERPINC1 (c.1377delC) -EF1-copGFP-T2A-Puro. (b) Schematic diagram of mutant c.685 T plasmid vector: pCDH-CMV-SERPINC1 (c.685C > T) -EF1-copGFP-T2A-Puro. (c) Electropherogram of wild-type plasmid digested with NotI/XhoI; (d) Electropherogram of mutant c.1377delC plasmid digested with NotI/XhoI; (e) Electropherogram of mutant c.685C > T plasmid digested with NotI/XhoI, Lane 1: undigested electropherogram, Lane 2: electropherogram after double digestion, Lane 3: DNA marker. (g-j) Sequencing of the clone plasmid of the SERPINC1 gene