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. 2023 Jan 9;21:3. doi: 10.1186/s12959-022-00443-6

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© The Author(s) 2023

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 3 — (a) Green fluorescent protein expression after transfection of cells carrying unloaded (Control), wild-type (SERPINC1-WT), Asn460Thrfs*20 mutant (SERPINC1-c.1377delC), and Arg229* mutant (SERPINC1-685C > T), suggesting that there was no significant difference in transfection efficiency among the groups. (b) Relative SERPINC1 mRNA expression levels (*, P < 0.05, * *, P < 0.01, * *, P < 0.005, * *, P < 0.001) in HEK293T cells by Control, SERPINC1-WT, Asn460Thrfs20 mutant (SERPINC1-c.1377delC), and Arg229* mutant (SERPINC1-685C > T). (c) Western blotting was used to detect the expression of AT protein in HEK293T cells by Control, SERPINC1-WT, Asn460Thrfs*20 mutant (SERPINC1-c.1377delC) and Arg229* mutant (SERPINC1-685C > T), respectively. (d) Based on the enzyme-linked immunosorbent assay (ELISA) standard curve, the expression of Control, SERPINC1-WT, Asn460Thrfs*20 mutant (SERPINC1-c.1377delC), and Arg229* mutant (SERPINC1-685C > T) in the supernatant of HEK293T cells was calculated. (*, P < 0.05, * *, P < 0.01, * *, P < 0.005, * *, P < 0.001)