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. 2023 Jan 9;13:4. doi: 10.1186/s13578-023-00954-3

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© The Author(s) 2023

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 7 — EF pre-stimulation increased neuronal differentiation of transplanted NSCs in impacted spinal cord. A–C. The transplanted EGFP-NSCs with EF pre-stimulation showed increased neurite process (A, B) and βIII-tubulin/EGFP co-localization (A, C) than those NSCs with NoEF treatment D, E. The transplanted EGFP-NSCs with EF pre-stimulation showed less GFAP/EGFP co-localization than those NSCs with NoEF treatment. Scale bars: 20 μm. * P < 0.05, ** P < 0.01 and *** P < 0.001 were considered as significantly different between EF and NoEF groups. n = 5 mice in EF and n = 6 mice in NoEF group