Fig. 3.

(A) NMDS analysis of bacterial communities based on the Bray-Curtis distance. (B) Absolute abundance of 16S rRNA gene in different sludge samples. Significant differences between 16S rRNA gene abundances among different treatments were tested (ANOVA, LSD test, P < 0.001). (C) Relative abundance of bacterial phyla. Others contains the taxa with an abundance of <0.01 in samples. (D) Procrustes test depicting the significant correlation between the ARG pattern (including MGEs) and bacterial ASV composition based on the Bray-Curtis dissimilarity metrics (sum of squares M² = 0.4626, r = 0.73, P < 0.001; 9999 permutations).