Fig. 3.

Effect of active compounds on PLC activity. (A) Representative images (left) and GFP fluorescence traces (right) showing relative changes in cytosolic GFP fluorescence in FRT cells stably expressing PH-PLCδ-GFP probe. During the assay, cells were sequentially stimulated with low (0.25 µM) and high (100 µM) UTP concentration. PLC activation, causing PIP₂ breakdown, results in detachment of the probe from the plasma membrane and redistribution to the cytosol. Vehicle or indicated compounds (10 µM) were added to the cells 20 min before the assay. (B) Summary of data showing the increase in cytosolic PH-PLCδ-GFP localization elicited by the addition of UTP (0.25 µM, top; 100 µM, bottom) in presence of vehicle or compounds. Where indicated, cells were pre-incubated with the membrane-permeable BAPTA/AM to chelate cytosolic Ca²⁺. ***P <0.001 vs. vehicle without BAPTA. ##P <0.01; ###P <0.001 vs. indicated condition. ns: not significant (ANOVA with Tukey’s post-hoc test).