Table 2.
Primer sequences for real-time quantitative PCR analysis
| Genes1 | Accession number | Primer sequence | Product size, bp |
|---|---|---|---|
| Claudin1 | NM_001013611.2 | F: GACCAGGTGAAGAAGATGCGGATG | 107 |
| R: CGAGCCACTCTGTTGCCATACC | |||
| Occludin | XM_025144248.1 | F: TCATCGCCTCCATCGTCTAC | 240 |
| R: TCTTACTGCGCGTCTTCTGG | |||
| ZO-1 | XM_015278981.2 | F: CTTCAGGTGTTTCTCTTCCTCCTC | 131 |
| R: CTGTGGTTTCATGGCTGGATC | |||
| ZO-2 | NM_204918.1 | F: GAGAGCACAACCGAAGCAGAGG | 157 |
| R: TAGTCCTGTCCATAGCCACCATCC | |||
| Mucin-2 | NM_001318434.1 | F: ACTGGACTTCACGGACACCT | 121 |
| R: CCCCCTCTACCATCATCAAA | |||
| IL-1β | XM_015297469.1 | F: GTACCGAGTACAACCCCTGC | 112 |
| R: AGCAACGGGACGGTAATGAA | |||
| IL-6 | NM_204628.1 | F: TGTGCAAGAAGTTCACCGTG | 213 |
| R: ACTCGACGTTCTGCTTTTCG | |||
| TNF-α | NC_006101.4 | F: GCACTCCGTTCAGACATCCA | 112 |
| R: CGCACCTGTCCTGTATCTGC | |||
| β-actin | NM_205518.1 | F: ATCCGGACCCTCCATTGTC | 120 |
| R: AGCCATGCCAATCTCGTCTT |
1ZO-1, zonula occludens-1; ZO-2, zonula occludens-2; IL-1β, interleukin-1β; IL-6, interleukin-6; TNF-α, tumor necrosis factor-α.