Figure 4.

MiR-146a KO promotes the synthesis of proinflammatory cytokines in skin wounds. (A) The IL-1β, TNF-α gene expression was tested by qRT-PCR in miR-146a WT, KO mice, diabetic miR-146a KO and WT mice (n = 3). (B) Protein levels of IL-1β, TNF-α were detected by western blot in different groups (n = 3). Values are given as mean ± SD of three individual experiments. Two-way ANOVA analysis was used to compare multiple groups (A, B). Statistical significance was determined as *p < 0.05. TNF-α, tumor necrosis factor alpha; qRT-PCR, quantitative reverse transcription PCR.