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. Author manuscript; available in PMC: 2023 Aug 8.
Published in final edited form as: Cancer Cell. 2022 Jul 21;40(8):818–834.e9. doi: 10.1016/j.ccell.2022.06.011

Figure 4. Oncogenic Col1 homotrimers produced by pancreatic cancer cells induce persistent proliferation signals.

Figure 4.

(A-C) RNA sequencing (RNA-seq) analysis on KPPC;Col1pdxKO cancer cells cultured on pre-coated vehicle, Col1 homotrimer, or Col1 heterotrimer (n = 3/group). Heatmap of differentially expressed genes was shown in (A). IPA was performed to visualize the most differentially regulated signaling network (B). CP: canonical pathway. GSEA revealed upregulated pathways by Col1 homotrimers as compared to Col1 heterotrimer (C).

(D) KPPC;Col1pdxKO cancer cells were treated with Col1 homotrimers or heterotrimers for 4–16 hours and examined for intracellular signaling by Western blotting. Representative Western blot of three independently experiments was shown.

See also Figures S3 and S4; Table S2.