KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Goat Collagen I | SouthernBiotech | Cat# 1310-01, RRID:AB_2753206 |
| Rabbit α3 integrin | Sigma-Aldrich | Cat# HPA008572, RRID:AB_2668094 |
| Mouse Pimonidazole | HPI | Cat# HP1-100, RRID:AB_2811309 |
| Goat Collagen IV | Abcam | Cat# ab6585, RRID:AB_305583 |
| Rabbit Thrombospondin-1 | Abcam | Cat# ab85762, RRID:AB_10674322 |
| Rabbit Laminin | Sigma-Aldrich | Cat# L9393, RRID:AB_477163 |
| Rabbit FSP1 | DAKO | Cat# A5114, RRID:AB_2335679 |
| Rabbit CD3 | Abcam | Cat# ab16669, RRID:AB_443425 |
| Rabbit CD4 | Cell Signaling | Cat# 25229, RRID:AB_2798898 |
| Rabbit CD8a | Cell Signaling | Cat# 98941, RRID:AB_2756376 |
| Rat Cytokeratin 8 | DSHB | Cat# TROMA-I, RRID:AB_531826 |
| Rat FoxP3 | eBioscience | Cat# 14-4771-80, RRID:AB_529583 |
| Rabbit phospho-ERK1/2 | Cell Signaling | Cat# 4376, RRID:AB_331772 |
| Rabbit ERK | Cell Signaling | Cat# 9102, RRID:AB_330744 |
| Rabbit phospho-AKT | Cell Signaling | Cat# 4060, RRID:AB_2315049 |
| Rabbit AKT | Cell Signaling | Cat# 4691, RRID:AB_915783 |
| Rabbit β-actin | Cell Signaling | Cat# 4970, RRID:AB_2223172 |
| Rabbit phospho-DDR1 | Cell Signaling | Cat#11994, RRID:AB_2797793 |
| Rabbit DDR1 | Cell Signaling | Cat# 5583, RRID:AB_10694842 |
| Rabbit phospho-FAK | Abcam | Cat# ab39967, RRID:AB_955850 |
| Rabbit FAK | Cell Signaling | Cat# 3285, RRID:AB_2269034 |
| Rabbit α1 integrin | Abcam | Cat# ab78479, RRID:AB_2280644 |
| Rabbit α2 integrin | Abcam | Cat# ab133557, RRID:AB_2833020 |
| Mouse α3 integrin | BD Biosciences | Cat# 611044, RRID:AB_2129599 |
| Mouse α3 integrin | Santa Cruz | Cat# sc-13545, RRID:AB_668044 |
| Rabbit β1 integrin | Santa Cruz | Cat# sc-8978, RRID:AB_2130101 |
| Rat PDGFRα(CD140a)-PE | BioLegend | Cat# 135905, RRID:AB_1953268 |
| Rat EpCAM(CD326)-AF488 | BioLegend | Cat# 118210, RRID:AB_1134099 |
| Rat CD45-Pacific Blue | BioLegend | Cat# 103126, RRID:AB_493535 |
| Rat CD3-AF700 | eBioscience | Cat# 56-0032-82, RRID:AB_529507 |
| Rat CD11b-BV711 | BD | Cat# 563168, RRID:AB_2716860 |
| Rat CD45RB-FITC | eBioscience | Cat# 11-0455-82, RRID:AB_465064 |
| Hamster CD3e PE-Cy7 | eBioscience | Cat# 25-0031-82, RRID:AB_469572 |
| Rat CD4-BV605 | Biolegend | Cat# 100548, RRID:AB_2563054 |
| Rat CD8a-BV650 | Biolegend | Cat# 100742, RRID:AB_2563056 |
| Mouse Granzyme B APC | Invitrogen | Cat# GRB05, RRID:AB_2536539 |
| Rat CD279 (PD-1) PerCP-Cy5.5 | Biolegend | Cat# 135208, RRID:AB_2159184 |
| Mouse CD25 APC-R700 | BD Biosciences | Cat# 659126, RRID:AB_2870475 |
| Hamster CD69 PE-CF594 | BD Biosciences | Cat# 562455, RRID:AB_11154217 |
| Hamster CD3e | BD Biosciences | Cat# 553057, RRID:AB_394590 |
| Hamster CD28 | BD Biosciences | Cat# 553294, RRID:AB_394763 |
| Rat CXCL16 | R&D Systems | Cat# MAB503, RRID:AB_2276752 |
| Rat CXCL5 | Novus Biological | Cat# NBP2-22026 |
| Rat PD-1 | Bio X Cell | Cat# BE0146, RRID:AB_10949053 |
| Bacterial and Virus Strains | ||
| Biological Samples | ||
| Tissue microarray sections of human PDAC | MDACC | IRB LAB05-0854 |
| Chemicals, Peptides, and Recombinant Proteins | ||
| Phosphatase inhibitor | Roche | Cat# 04906845001 |
| Protease inhibitor | Roche | Cat# 4693116001 |
| SB 225002 | Tocris | Cat# 2725/10 |
| Collagenase IV | Gibco | Cat# 17104019 |
| Dispase II | Gibco | Cat# 17105041 |
| Liberase | Roche | Cat# 5401020001 |
| DNase I | Roche | Cat# 10104159001 |
| Type I collagen solution from rat tail | Sigma-Aldrich | Cat# C3867 |
| Critical Commercial Assays | ||
| Direct-zol RNA Kit | Zymo Research | Cat# R2050 |
| Reverse Transcription Kit | Applied Biosystems | Cat# 4368814 |
| SYBR Green Master Mix | Applied Biosystems | Cat# 4367659 |
| Cell Counting Kit-8 | Abcam | Cat# ab228554 |
| Live-Dead-eFluor780 | eBioscience | Cat# 65-0865-14 |
| Foxp3/Transcription Factor Staining Buffer Set | eBioscience | Cat# 00-5523-00 |
| Gomori’s Trichrome Stain Kit | Leica Biosystems | Cat# 38016SS2 |
| Chromium Single Cell 3’ Reagent Kits (v2) | 10x Genomics | Cat# PN-120237 |
| ABC-Kit | Vector | Cat# PK-6100 |
| Stable DAB | Invitrogen | Cat# 750118 |
| Direct Red 80 | Sigma-Aldrich | Cat# 365548 |
| Pierce BCA protein assay kit | Thermo Fisher | Cat# 23208 |
| Sircol collagen assay kit | Biocolor | Cat# S1000 |
| TruSeq Stranded mRNA Sample Prep Kit | Illumina | Cat# 20020594 |
| Deposited Data | ||
| KPPC and KPPC;Col1pdxKO tumor RNA-sequencing | This paper | GEO: GSE131357 |
| KPPC and KPPC;Col1pdxKO cell RNA-sequencing | This paper | GEO: GSE131064 |
| Col1 treated cancer cells RNA-sequencing | This paper | GEO: GSE131666 |
| Single-cell RNA-sequencing of mouse PDAC tumors | Chen et al., 2021 | GEO: GSE166298 |
| Single-cell RNA-sequencing of human PDAC tumors | Peng et al., 2019 | GSA: CRA001160 |
| TCGA pancreatic adenocarcinoma cohort survival and gene expression data (GDAC Firehose PAAD) | Broad Institute | http://gdac.broadinstitute.org/runs/stddata__2016_01_28/data/PAAD/20160128/ |
| Experimental Models: Cell Lines | ||
| Primary mouse KPPC cancer cell lines | This paper | N/A |
| Primary mouse KPPC;Col1pdxKO cancer cell lines | This paper | N/A |
| Experimental Models: Organisms/Strains | ||
| Mouse: LSL-KrasG12D/+;Pdx1-Cre | Hingorani et al., 2005 | N/A |
| Mouse: Trp53loxP/+ | Chen et al., 2005 | N/A |
| Mouse: Col1a1loxP/loxP | Chen et al., 2021 | N/A |
| Oligonucleotides | ||
| See Table S5 | ||
| Recombinant DNA | ||
| Software and Algorithms | ||
| Flowjo v10.7.1 | Flowjo, L.L.C. | RRID: SCR_008520 |
| Prism v8.0.0 | GraphPad Software Inc. | RRID: SCR_002798 |
| Fiji v2.0.0 | ImageJ | RRID: SCR_002285 |
| cBioportal v2.2.0 | MSK Center for Mol Onc | https://www.cbioportal.org/ |
| Seurat R package (3.5.3) | Satija et al., 2015 | https://satijalab.org/seurat/ |
| DESeq2 | Anders and Huber, 2010 | https://bioconductor.org/packages/release/bioc/html/DESeq2.html |
| STAR aligner | Dobin et al., 2013 | https://github.com/alexdobin/STAR |
| Gene Set Enrichment Analysis (GSEA) | Broad Institute | http://software.broadinstitute.org/gsea/index.jsp |
| Other | ||