FIGURE 3.

Involvement of reactive oxygen species in the αMMC‐regulated defence response to CMV. (a) H₂O₂ level evaluated by 3,3′‐diaminobenzidine (DAB) staining. (b) Superoxide level evaluated by nitroblue terazolium (NBT) staining. Five plants were used for each treatment; a picture of one representative leaf is shown. (c) Quantitative detection of H₂O₂ content. (d) Quantitative measurements of malondialdehyde (MDA) content. Determination of (e) relative water content (RWC) and (f) electrolyte leakage in wild‐type (WT) plants and αMMC transgenic plants. Systemic leaves were harvested for analysis. Values indicate the means ± SD from three independent experiments and lowercase letters represent significant differences (p < 0.05). FW, fresh weight