FIGURE 5.

αMMC interacts with catalases. (a) Bimolecular fluorescence complementation analysis of the interactions among catalases, αMMC, and 2b in Nicotiana benthamiana. Green fluorescence illustrates the interaction, bars = 40 μm. (b) Yeast two‐hybrid assay. Yeast cells grown on QDO medium lacking Leu, Trp, His, and Ade (−LWHA) suggested the interaction. AD, GAL4‐activation domain. BD, GAL4 DNA‐binding domain. (c) Pull‐down assay results suggesting that αMMC interacted with CAT1/CAT2 in vitro. The purified CAT1‐GST, CAT2‐GST and glutathione‐S‐transferase (GST) proteins were each incubated with αMMC‐MBP. After immunoprecipitation with maltose‐binding protein (MBP) beads, the proteins were monitored by anti‐MBP or anti‐GST antibodies using western blotting. Red asterisks indicate bands of the target protein