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. 2022 Nov 20;24(2):123–141. doi: 10.1111/mpp.13280

FIGURE 1.

FIGURE 1

Identification and expression analysis of novel stilbene synthase transcripts in Vitis quinquangularis accession Danfeng‐2. (a) Expression analysis of novel transcripts in V. quinquangularis accession Danfeng‐2. Novel transcripts are grouped into 11 clusters. The numbers of transcripts from clusters 1 to 11 were 311, 322, 76, 53, 205, 380, 324, 460, 385, 328, and 6600, respectively. Expression of transcripts in cluster 11 was the highest at the ripe stage. (b) Analysis of the expression profiles of novel stilbene synthase transcripts in Danfeng‐2. Six stilbene synthase transcripts were isolated from cluster 11, with locus names c50176.graph_c0, c91313.graph_c0, c76190.graph_c0, c56362.graph_c0, c54055.graph_c0, and c11357.graph_c0. The four developmental stages of Danfeng‐2 berries were represented by DF_GH (green hard stage, 25 days after blooming), DF_BV (before véraison stage, 40 days after blooming), DF_V (véraison stage, 50 days after blooming), and DF_R (ripe stage, 80 days after blooming). (c) Multiple sequence alignment of VqNSTS1–6 amino acid sequences. The red rectangles represent the two conserved motifs. VqNSTS1 is a pseudogene, and VqNSTS5/6 contain mutations in the conserved motif. (d) The phylogenetic tree of VqNSTS1–6 with 41 reported VqSTSs and 48 reported VvSTSs. The 41 reported VqSTSs are from Danfeng‐2. All 48 reported VvSTSs are from V. vinifera ‘Pinot Noir’. They were divided into six subgroups: VqNSTS1, VqNSTS3, VqNSTS5, and VqNSTS6 belong to subgroup II; VqNSTS2 belongs to subgroup V; and VqNSTS4 belongs to subgroup I. (e–i) Reverse transcription‐quantitative PCR (RT‐qPCR) analysis was conducted to determine the relative transcript levels of VqNSTS2–6 after inoculation with Uncinula necator. VqNSTS2/4 showed high expression after U. necator inoculation, especially VqNSTS4. Asterisks indicate significant differences (*p < 0.05, **p < 0.01, Student's t test). (j–n) RT‐qPCR analysis was conducted to determine the relative transcript levels of VqNSTS2–6 after treatment with 100 μM abscisic acid (ABA), ethylene (Eth), salicylic acid (SA), or methyl jasmonate (MeJA). VqNSTS4 responded significantly to SA treatment. The standard deviation (SD) was calculated from three independent replicates. One‐way analysis of variance (Tukey's test) was carried out, with asterisks indicating significant differences at *p < 0.05, **p < 0.01.