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. 2022 Nov 20;24(2):123–141. doi: 10.1111/mpp.13280

FIGURE 5.

FIGURE 5

Transient expression of transcription factor VqAL4 in Vitis quinquangularis accession Danfeng‐2 promoted transcription of VqNSTS4 and antifungal stilbene accumulation. (a) Schematic diagram of overexpression and RNAi vectors. (b, c) Transient transformation of 35S‐VqAL4‐GFP and RNAi‐VqAL4‐GFP in Danfeng‐2 leaves using the Agrobacterium vacuum infiltration method. (d) Western blot assays were carried out to determine the expression of 35S‐VqAL4‐GFP in transiently transformed Danfeng‐2 leaves. EV, the empty vector pCAMBIA2300‐35S‐GFP was used as the control. (e) Western blot assays were carried out to determine the expression of RNAi‐VqAL4‐GFP in transiently transformed Danfeng‐2 leaves. EV, the empty vector pK7GWIWG2(II)‐35S‐GFP was used as the control. (f, i) Reverse transcription‐quantitative PCR analysis was conducted to determine the relative expression levels of VqAL4 and VqNSTS4 in transiently transformed Danfeng‐2 leaves. (g, j) Determination of five stilbene contents in transiently transformed Danfeng‐2 leaves. (h, k) Free salicylic acid (SA) content in transiently transformed Danfeng‐2 leaves. The standard deviation (SD) was calculated from three independent replicates. One‐way analysis of variance (Tukey's test) was carried out. Asterisks indicate significant differences at *p < 0.05, **p < 0.01.