Skip to main content
. 2022 Nov 20;24(2):123–141. doi: 10.1111/mpp.13280

FIGURE 8.

FIGURE 8

Transient silencing of VqAL4 in leaves of Danfeng‐2 reduces resistance to Uncinula necator. (a) Phenotypes of control EV and transiently silenced RNAi‐VqAL4‐GFP Danfeng‐2 leaves at 1 day postinoculation (dpi) and 7 dpi. EV, the empty vector pK7GWIWG2(II)‐35S‐GFP. (b) Trypan blue staining of leaves from EV and transiently silenced RNAi‐VqAL4‐GFP leaves at 1 dpi and 7 dpi. Bars = 100 μm. (c) Quantification of spores per mg fresh leaves from EV and transiently silenced RNAi‐VqAL4‐GFP leaves at 7 dpi. (d) Free salicylic acid (SA) content in the leaves of EV and transiently silenced RNAi‐VqAL4‐GFP leaves at 7 dpi. (e–g) Reverse transcription‐quantitative PCR analysis was conducted to determine the relative transcript levels of SA‐related genes in EV and transiently silenced RNAi‐VqAL4‐GFP leaves following U. necator inoculation. The standard deviation (SD) was calculated from three independent replicates. One‐way analysis of variance (Tukey's test) was carried out. Asterisks indicate significant differences at *p < 0.05, **p < 0.01.