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. 2023 Jan 4;111(1):92–105.e5. doi: 10.1016/j.neuron.2022.10.008

Figure 2.

Figure 2

Molecular characterization of Kcnip2GlyT2 neurons in the spinal cord and DRG

(A) Recombination strategy for Kcnip2 GlyT2 intersectional targeting. Ct-Cre and Nt-cre denote C- and N-terminal Cre fragments.

(B) Transverse section of lumbar spinal cord of Kcnip2roxCre;GlyT2::Dre;ROSA26lsl-tdTom triple transgenic mice. Red, Kcnip2 neurons. Scale bar, 100 μm.

(C) Multicolor in situ hybridization. mRNA expression of tdTom (red), Kcnip2 (green), and vGAT (gray). DAPI (4′,6-diamidino-2-phenylindole) staining shown in blue, n = 11 sections from two mice. Scale bar, 20 μm. Mean ± SEM.

(D) No tdTom neurons were found in lumbar DRGs. Scale bar, 100 μm.