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. 2023 Jan 10;13:519. doi: 10.1038/s41598-023-27737-7

Figure 2.

Figure 2

AT1a receptor loss reduced the gene expressions related to protein degradation system through downregulation of FoxO1 expression in the denervated gastrocnemius muscle. (a, b) Gene expressions of MuRF1 (a) and Atrogin-1 (b) were evaluated by RT-qPCR. Expression levels of these transcripts in each sample were normalized to 18S rRNA expression levels and were shown as the fold increase or decrease in mRNA expression in the Den group compared with the Cont group. (c–f) Protein expression levels of phosphorylated FoxO1 and total FoxO1 were evaluated by western blot analysis. Expression levels in each sample were normalized to α-tubulin expression levels and were shown as the fold increase or decrease in the protein expression in the Den group compared with the Cont group. Relative protein expression was calculated for p-FoxO1/FoxO1 (d), p-FoxO1/α-tubulin (e), FoxO1/α-tubulin (f). Samples from the same experiment were processed in parallel for SDS-PAGE and western blotting using different gels and membranes, and the image data obtained were cropped. Entire images of western blotting are shown in online supplementary resource Supplementary Fig. S5. AT1a+/+-Cont group, n = 6; AT1a+/+-Den group at 7 days postdenervation, n = 7; AT1a+/+-Den group at 21 days postdenervation, n = 7; AT1a−/−-Cont group, n = 5; AT1a−/−-Den group at 7 days postdenervation, n = 6; AT1a−/−-Den group at 21 days postdenervation,n = 6. Values are means ± SE. *P < 0.05, **P < 0.01.