Fig. 1.

SOD1^G93A constitutively released from NSC-34 motor neurons induces ER stress in recipient NSC-34 motor neurons. A–C XBP1-tdTomato-transfected NSC-34 cells were incubated with pelletable fractions from conditioned media of non-transfected (NT), or EGFP- or SOD1^G93A-EGFP (SOD1^G93A)-transfected NSC-34 cells. At 24 h, A the percentage of EGFP⁺ cells was assessed by flow cytometry, B cellular XBP1-tdTomato fluorescence was visualised by an IncuCyte ZOOM Live-cell Analysis System, and C the MFI of XBP1-tdTomato in cells was assessed by flow cytometry. A, C Data are presented as mean ± SD (n = 4); **p < 0.02, ***p < 0.01 compared to NT, ^†p < 0.05, ^†††p < 0.01 compared to EGFP. B Data are representative of n = 4 experiments; bars represent 300 µm