Fig. 3.

NSC-34 motor neurons express functional P2X7. A P2X7 expression in whole lysates of EOC13, murine P2X7-transfected HEK-293 (mP2X7) and NSC-34 cells was assessed by immunoblotting using an anti-P2X7 antibody. Data is representative of n = 3 experiments. B–D Non-transfected (NT) or D SOD1^G93A-EGFP (SOD1^G93A)-transfected NSC-34 cells were pre-incubated in the B–D absence or C presence of 10 μM AZ10606120 (AZ10) for 1 h. Cells were then incubated with 25 μM ethidium bromide in the absence (basal) or presence of 5 mM ATP for 20 min, and the reactions were stopped by the addition of 20 mM MgCl₂. The MFI of ethidium⁺ uptake into cells was assessed by flow cytometry. Data are presented as mean ± SD (n = 3); ***p < 0.01 compared to corresponding basal, ^†††p < 0.01 compared to ATP in the absence of AZ10