FIG. 2.

Immunoblot of eukaryotic cells with polyclonal anti-OprF mouse sera. Lanes (labeled as follows): Cells, lysed UM449 cells that had been transfected with pVR1020/oprF, incubated for 48 h, washed in PBS, and then resuspended in PBS for lysis; Sup., the initial 48-hour culture supernatant from UM449 cells transfected with pVR1020/oprF; and Control, lysed UM449 cells transfected with pVR1020. Note that OprF was seen predominantly in the pelleted cell fraction but was also found in the supernatant.