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. 2022 Dec 1;13:1071394. doi: 10.3389/fmicb.2022.1071394

Figure 2.

Figure 2

miR-615 facilitates PEDV infection in MARC-145 cells and IECs. (A and B) PEDV regulated miR-615 expression in IECs and MARC-145 cells. IECs (A) and MARC-145 cells (B) were infected with PEDV at a multiplicity of infection (MOI) of 1 and samples were collected at 6, 12, and 24 h post-infection. RT-qPCR was used to assess miR-615 expression. (C–F) IECs and MARC-145 cells were transfected with the MC, miR-615 mimic, IC, or miR-615 inhibitor (100 nmol). After 24 h, the cells were infected with PEDV at an MOI of 1. After 18 h, the PEDV genomic RNA of the MC, miR-615 mimic, IC, and miR-615 inhibitor in the IECs (A,B) and transfected MARC-145 cells (C,D) were assessed using RT-qPCR. PEDV N protein level was measured using western blotting following transfection with the MC or miR-615 mimic for 6 and 12 h in the IECs (G) and MARC-145 cells (H). The intensity represents PEDV N protein levels normalized against that of β-actin across three independent experiments in IECs and MARC-145 cells, respectively. The data are presented as the mean ± SD of three independent experiments, performed with technical duplicates. *p<0.05, **p < 0.01, ***p < 0.001.